Protein Cleavage

Breaking a peptide bond is done via hydrolysis. Water is used to break the bond. Water was produced when the bond was formed.

"lysis" means separation

Acid hydrolysis

Non specific - any peptide bond will break. Heat the up protein.

Proteolysis

Protease enzymes will break bonds at specific sites. e.g. trypsin will break peptides on the carboxyl side of lysine or arginine.

Peptide bonds: Formation and cleavage